Determination of a protein ligand interaction via continuous isothermal titration calorimetry
نویسنده
چکیده
Isothermal titration calorimetry (ITC) is a straightforward method to determine basic chemical details of a binding interaction (affinity, thermodynamics and stoichiometry) in a single experiment and under native conditions [1, 2]. Traditionally, ITC experiments are performed using the method of incremental titration, whereby a precise volume of titrant is added to a solution of titrand at discrete time-intervals (Figure. 1). The area under each peak is then integrated, normalized and fit to a model to calculate affinity, enthalpy and stoichiometry for the interaction.
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